The human BCL2 gene was the first anti-death gene discovered. 5īCL2 orthologs have been found in many mammalian species for which deciphered genomes are available. It is a mitochondrial membrane protein of 25,000 molecular mass that can block programmed cell death and, when dysregulated, can extend the survival of many cell lineages, especially in hematopoietic tissues. It is expressed in most embryonic and many adult tissues, but particularly in B and T cells. The prototype family member, B-Cell Lymphoma 2 (BCL2), is a powerful inhibitor of cell death. In addition, some members are involved in the activation of members of the caspase family of apoptosis enzymes. 4 At least some family members are believed to elicit release of cytochrome C from mitochondria and subsequent cell death. They comprise proteins that facilitate apoptosis, as well as apoptosis inhibitors. Members of the BCL2 family of proteins play important roles in the control of programmed cell death (apoptosis). In addition to revealing breaks, which lead to translocation of parts of the gene or its fusion to other genes, the probe set can also be used to identify other BCL2 aberrations such as deletions, amplifications or chromosome 18 hyperdiploidy.įluorescence in situ hybridization (FISH) is a powerful technique developed to detect presence or absence, location, integrity and amount of genomic sequences in tissue samples or cells. The two probes are designed to recognize sequences on both sides of an area inside the BCL2 gene where breakpoints are frequently found. The kit contains two differentially labeled Locus Specific Probes (LSP), one covering the 5’ (start) portion of the BCL2 gene and some upstream untranslated genomic sequence, the other covering sequences downstream of the 3’ (end) part of the gene. The BCL2 FISH probe set is designed to detect rearrangements involving regions of the human BCL2 gene located on chromosome band 18q21. Publications ♼ontact SupportīCL2 Break Apart FISH Probe Kit Cat No CT-PAC206.
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